Kinetic Studies of Human Carbonic Anhydrases B and C.
نویسندگان
چکیده
منابع مشابه
Kinetic Studies of Human Carbonic Anhvdrases B and C*
Carbonic anhydrase preparations from human erythrocytes contain at least three distinct molecular forms of the enzyme (l-4), two of which-denoted here as carbonic anhydrases B and C-have been studied in detail. They differ widely in specific activity, and also in their isoelectric points (1, 4), their amino acid composition (4-6), and their chromatographic behavior (3,4). Earlier studies on the...
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Detailed studies of a relatively large number of human erythrocyte carbonic anhydrases of different electrophoretic mobilities have shown that individually each is very similar to one of the major isozymes, either B or C. The enzymatic activities, the zinc, nitrogen, and amino acid contents, and the molecular weights of members of a given type are similar. Some differences in the tryptic peptid...
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The more acidic, minor isozymes of human carbonic anhydrase types B and C are present in higher concentrations in the older erythrocyte populations. The levels of all of these isozymes can be determined quantitatively by immunochemical methods following their separation by electiofocusing. A wide variety of human tissues have been assayed for carbonic anhydrases. Significant levels of the C typ...
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The prototype of the gamma-class of carbonic anhydrase has been characterized from the methanogenic archaeon Methanosarcina thermophila. Previously reported kinetic studies of the gamma-class carbonic anhydrase are consistent with this enzyme having a reaction mechanism similar to that of the mammalian alpha-class carbonic anhydrase. However, the overall folds of these two enzymes are dissimila...
متن کاملPurification and properties of human erythrocyte carbonic anhydrases.
Three different methods are described for separating hemoglobins from carbonic anhydrases in hemolysates from human erythrocytes. The preferred method involves adsorption on diethylaminoethyl Sephadex at pH 8.7 followed by selective elution of the carbonic anhydrases. Carbonic anhydrases A, B, and C are subsequently separated from each other on DEAE-Sephadex by elution with 0.05 M Trischloride ...
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 239 شماره
صفحات -
تاریخ انتشار 1964